
Gene Overexpression
The service requires customers to provide the followings(details in the order system):
(1) Lentiviral vector plasmid that has been constructed or to commission my company to build
(2) Sequencing report of the target gene
(3) Sequence of lentiviral vector, vector map, and the like
(4) The relevant primers need to be provide for sequencing
(5) The amount of virus (TU) and the virus titer range
(6) In addition, if you have special requirements about the amount of virus, please contact us directly.
Company need provide customers with the following information:
(1) Vector construction report and sequencing report after building to the carrier;
(2) Lentiviral production reports;
(3) Reports of lentivirus concentration, purification and titer titration;
(4) Full detailed lab report and raw data
Overexpression Experiments Shuttle Plasmid
Vectors |
Promoters |
Tags |
GeneInsertionSites |
Notes |
TG001 |
hPGK |
EGFP |
BamH1/Sal1 |
|
TG002 |
hPGK |
No |
MCS |
Polylinker (MCS) |
TG005 |
EF1a |
EGFP |
Mlu1/spe1 |
GFP-IRES-gene |
TG006 |
EF1a |
EGFP |
Blunt-endligation |
Gene-IRES-GFP |
TG007 |
Tetracycline-inducible promoter/Liver-specific promoter |
Luciferase/GFP |
choice |
IRES |
TG008 |
CMV/SV40 |
GFP/Neo |
choice |
The dual promoter high expression sieve stable strains
|