Gene Overexpression

The service requires customers to provide the followings(details in the order system):
(1) Lentiviral vector plasmid that has been constructed or to commission my company to build
(2) Sequencing report of the target gene
(3) Sequence of lentiviral vector, vector map, and the like
(4) The relevant primers need to be provide for sequencing
(5) The amount of virus (TU) and the virus titer range
(6) In addition, if you have special requirements about the amount of virus, please contact us directly.
 

Company need provide customers with the following information:
(1) Vector construction report and sequencing report after building to the carrier;
(2) Lentiviral production reports;
(3) Reports of lentivirus concentration, purification and titer titration;
(4) Full detailed lab report and raw data


Overexpression Experiments Shuttle Plasmid

Vectors

Promoters

Tags

GeneInsertionSites

Notes

TG001

hPGK

EGFP

BamH1/Sal1

 

TG002

hPGK

No

MCS

Polylinker

(MCS)

TG005

EF1a

EGFP

Mlu1/spe1

GFP-IRES-gene

TG006

EF1a

EGFP

Blunt-endligation

Gene-IRES-GFP

TG007

Tetracycline-inducible promoter/Liver-specific promoter

Luciferase/GFP

choice

IRES

TG008

CMV/SV40

GFP/Neo

choice

The dual promoter high 

expression sieve stable strains